Sequence specific dna recombination in eukaryotic cells

Details Sequence specific dna recombination in eukaryotic cells Sequence specific dna recombination in eukaryotic cells

C - Chemistry, Metallurgy

12

N

C12N 15/90 (2006.01)

Patent

CA 2504010

The present invention relates to a method of sequence-specific recombination of DNA in eukaryotic cells, comprising the introduction of a first DNA comprising a nucleotide sequence containing at least one recombination sequence into a cell, introducing a second DNA comprising a nucleotide sequence containing at least one further recombination sequence into a cell, and performing the sequence specific recombination by a bacteriophage lambda integrase Int.

L'invention concerne un procédé de recombinaison d'ADN spécifique à une séquence dans des cellules eucaryotes. Le procédé consiste à introduire un premier ADN comprenant une séquence nucléotidique contenant au moins une séquence de recombinaison dans une cellule, à introduire un second ADN comprenant une séquence nucléotidique comprenant au moins une autre séquence de recombinaison dans une cellule, et à effectuer une recombinaison spécifique à la séquence, au moyen d'une intégrase Int <i>lambda </i>bactériophage.

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