Sequence specific dna recombination in eukaryotic cells

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/90 (2006.01) A01K 67/027 (2006.01) A61K 31/7088 (2006.01) A61K 48/00 (2006.01) C12N 5/10 (2006.01) C12N 15/86 (2006.01)

Patent

CA 2390526

The present invention relates to a method of sequence specific recombination of DNA in eukaryotic cells, comprising the introducing of a first DNA sequence into a cell, introducing a second DNA sequence into a cell, and performing the sequence specific recombination by a bacteriophage lambda integrase Int. A preferred embodiment of the invention relates to a method, further comprising performing the sequence specific recombination of DNA by an Int and a Xis factor. The present invention further relates to vectors and their use as medicaments.

L'invention concerne un procédé de recombinaison d'ADN séquence spécifique dans des cellules eucaryotes, qui comprend les étapes suivantes : introduire une première séquence d'ADN dans une cellule ; introduire une seconde séquence d'ADN dans une cellule, et recombiner de manière séquence-spécifique en faisant agir une intégrase-lambda Int bactériophage. Une variante préférée de l'invention concerne un procédé selon lequel, il est prévu en outre une seconde recombinaison d'ADN séquence-spécifique en faisant agir une Int et un facteur-Xis. L'invention concerne par ailleurs des vecteurs et leur utilisation comme médicaments.

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