Sequence-specific large volume sample preparation method and...

C - Chemistry – Metallurgy – 12 – Q

Patent

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C12Q 1/68 (2006.01) C12N 1/06 (2006.01) C12N 15/10 (2006.01)

Patent

CA 2750338

Methods of selectively and rapidly identifying target nucleic acid molecules in large volumes of collection media where the target is present in a low concentration are disclosed. The methods can be used to identify, isolate, purify, or enrich a nucleic acid molecule containing a specific target sequence from a sample of nucleic acid molecules that do not contain the specific target sequence. Once isolated, the nucleic acid molecule containing a specific target sequence may be amplified or used in a variety of detection assays.

L'invention concerne des procédés d'identification sélective et rapide de molécules d'acides nucléiques cibles dans de grands volumes de milieu de recueillement dans lesquels la cible est présente en une faible concentration. Les procédés peuvent être utilisés pour identifier, isoler, purifier ou enrichir une molécule d'acide nucléique contenant une séquence cible spécifique à partir d'un échantillon de molécules d'acides nucléiques qui ne contiennent pas la séquence cible spécifique. Une fois isolée, la molécule d'acide nucléique contenant une séquence cible spécifique peut être amplifiée ou utilisée dans divers dosages de détection.

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