C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/85 (2006.01) C07K 14/435 (2006.01) C07K 14/535 (2006.01) C12N 5/10 (2006.01) C12N 9/18 (2006.01) C12N 15/62 (2006.01)
Patent
CA 2360847
An expression cassette is disclosed which is useful for the secretion of a heterologous protein from mammalian and insect cells. The expression cassette comprises a polynucleotide sequence encoding a secretion competent polypeptide which is linked in frame to a heterologous gene sequence. Also disclosed is a method of secreting heterologous proteins in mammalian and insect cells using the expression cassette.
La présente invention concerne une cassette d'expression servant à la sécrétion d'une protéine hétérologue de cellules de mammifères et d'insectes. La cassette d'expression comprend une séquence polynucléotidique codant pour un polypeptide capable de sécrétion lié en phase à une séquence génique hétérologue. L'invention concerne également une méthode de sécrétion de protéines dans des cellules de mammifères et d'insectes à l'aide de la cassette d'expression.
Behie Leo A.
Farrell Patrick J.
Iatrou Kostas
Marks & Clerk
University Technologies International Inc.
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