Sequencing by hybridisation

Details Sequencing by hybridisation Sequencing by hybridisation

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01)

Patent

CA 2323110

A method of analysing a nucleic acid involves the use of a mixture of labelled oligonucleotides in solution and an array of immobilised oligonucleotides. The target nucleic acid is incubated with the mixture of labelled oligonucleotides. Those labelled oligonucleotides which hybridise are recovered and incubated with the array of immobilised oligonucleotides. Sequence information is obtained by observing the location of the label on the array. The method is particularly suitable for determining differences between nucleic acids.

La présente invention concerne l'analyse d'un acide nucléique avec utilisation d'un mélange d'oligonucléotides marqués en solution et d'une matrice d'oligonucléotides immobilisés. L'acide nucléique cible est mis à incuber avec le mélange d'oligonucléotides marqués. On récupère ceux des oligonucléotides marqués qui réalisent l'hybridation et on les met à incuber avec la matrice d'oligonucléotides immobilisés. Pour obtenir l'information de séquence, on observe l'emplacement des marqueurs sur la matrice. Ce procédé convient particulièrement pour la détermination de différences entre acides nucléiques.

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