Sequencing by ligation of encoded adaptors

Details Sequencing by ligation of encoded adaptors Sequencing by ligation of encoded adaptors

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01) C07H 21/00 (2006.01)

Patent

CA 2256700

The invention provides a method of nucleic acid sequence analysis based on the ligation of one or more sets of encoded adaptors to the terminus of a target polynucleotide. Encoded adaptors whose protruding strands form perfectly matched duplexes with the complementary protruding strands of the target polynucleotide are ligated, and the identity of the nucleotides in the protruding strands is determined by an oligonucleotide tag carried by the encoded adaptor. Such determination, or "decoding" is carried out by specifically hybridizing a labeled tag complement to its corresponding tag on the ligated adaptor.

La présente invention concerne un procédé d'analyse de séquences d'acide nucléique à partir de la ligature à l'extrémité d'un polynucléotide cible d'une ou plusieurs séries d'adaptateurs codés. Les adaptateurs codés, dont les brins saillants forment des duplex parfaitement concordants avec les brins saillants complémentaires du polynucléotide cible, sont soudés et l'identité des nucléotides des brins saillants est déterminée par un oligonucléotide marqueur porté par l'adaptateur codé. Cette identification ou "décodage" est réalisée par hybridation spécifique d'un complément de marqueur marqué avec son marqueur correspondant sur l'adaptateur soudé.

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