C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/68 (2006.01) C12M 1/00 (2006.01) C12M 1/34 (2006.01) C12P 19/34 (2006.01) G01N 33/48 (2006.01) G01N 33/50 (2006.01) G06F 19/00 (2006.01)
Patent
CA 2412973
In a method of identifying an unknown nucleotide sequence using base addition, a sequence of bases is obtained from a template, a base in the sequence is identified as an unknown base, an "unknown" indicator is included in the sequence, and an output sequence is generated containing the unknown base indicator. The sequence of bases is obtained from the template by evaluation of a reporter and assigning the bases in accordance therewith. A determination is made as to whether the reporter is from a preceding cycle of base determination, and if the reporter is from a preceding cycle of base determination, the base assignation is discarded.
L'invention concerne un procédé d'identification d'une séquence de nucléotides inconnue, qui permet, à l'aide d'addition de bases, d'obtenir une séquence de bases à partir d'une matrice et d'identifier une base dans la séquence comme base inconnue. Un indicateur <= inconnu >= est inclus dans la séquence, et une séquence de sortie est générée qui contient l'indicateur de base inconnue. La séquence de bases est obtenue à partir de la matrice par évaluation d'un reporter et en affectant les bases de manière correspondante. Le procédé consiste ensuite à déterminer si le reporter provient d'un cycle de détermination de base précédent, et si tel est le cas, l'affectation de base est supprimée.
Odedra Raj
Amersham Biosciences Uk Limited
Fetherstonhaugh & Co.
Ge Healthcare Uk Limited
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