C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/68 (2006.01) C09B 23/00 (2006.01) C12Q 1/70 (2006.01) G01N 21/64 (2006.01) G01N 27/447 (2006.01)
Patent
CA 2143559
To reduce the difficulties of obtaining absorbance and emission bands that reduce the effects of background noise during gel electrophoresis when irradiating with a diode laser scanning, there is provided a method of identifying strands of DNA comprising the steps of marking the strands with fluorescent labels that emit light in a region of wavelengths including at least one wavelength within the infrared and near infrared region wherein the fluorescent label includes a chromophore having the formula: Image or Image or Image where X is (CH2)n; n = 4-10 or X is -CH2 - CH2 - 0 - CH2 - CH2 - 0 - CH2 - CH2 -; or Image wherein X consists of one of O or NH, Y consists of one of NCS or H; and R consists of one of H, NCS, CH2OH, CH2NCS, COOH, irradiating the strands with light having a wavelength within one of the infrared and near infrared regions; and detecting the light emitted from the fluorescent labels. There is also provided a dye having the above formula.
Lipowska Malgorzata
Middendorf Lyle Richard
Narayan Narasimhachari
Patonay Gabor
Strekowski Lucjan
Li-Cor Inc.
Ridout & Maybee Llp
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