Sequencing of large nucleic acid fragments

Details Sequencing of large nucleic acid fragments Sequencing of large nucleic acid fragments

G - Physics

01

N

G01N 27/447 (2006.01) C12Q 1/68 (2006.01) G01N 27/26 (2006.01)

Patent

CA 2074282

The base sequence of large single stranded nucleic acids is determined by retarding the migration rate of a nucleic acid fragment of n bases below a migration rate which would otherwise be the same as the migration rate of a nucleic acid fragment of n bases in a polyacrylamide gel under continuous field gel electrophoresis. A plurality of sequences of electric field pulses is applied to the gel in one dimension.

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