Sequencing of nucleic acid molecules by mass spectrometry

Details Sequencing of nucleic acid molecules by mass spectrometry Sequencing of nucleic acid molecules by mass spectrometry

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01)

Patent

CA 2741959

The present invention is related to a method for determining the nucleotide sequence of a nucleic acid molecule comprising the following steps: a) providing a plurality of molecules of the nucleic acid molecule having at least one modification; b) cleaving at random the plurality of modified nucleic acid molecules thus providing modified nucleic acid molecule fragments and non-modified nucleic acid molecule fragments; c) separating the modified nucleic acid molecule fragments from the non-modified nucleic acid molecule fragments; d) separating or resolving the modified nucleic acid molecule fragments according to their length, mass and/or charge, whereby such separating or resolving generates a pattern of modified nucleic acid fragments; and e) optionally visualizing the pattern of modified nucleic acid fragments.

L'invention concerne une méthode de détermination de la séquence de nucléotides dune molécule dacide nucléique comprenant les étapes suivantes: a) obtention de molécules dacides nucléiques présentant au moins une modification; b) clivage aléatoire des molécules dacides nucléiques modifiées pour obtenir des fragments de molécules dacides nucléiques modifiées et des fragments de molécules dacides nucléiques non modifiées; c) séparation des fragments de molécules dacides nucléiques modifiées davec les fragments de molécules dacides nucléiques non modifiées; d) séparation ou résolution des fragments de molécules dacides nucléiques modifiées: par longueur, par masse et/ou par charge, de manière à créer un motif de fragments de molécules dacides nucléiques modifiées; et e) facultativement, visualisation du motif de fragments de molécules dacides nucléiques modifiées.

Affiliated with

Also associated with

Rating

Sequencing of nucleic acid molecules by mass spectrometry does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Sequencing of nucleic acid molecules by mass spectrometry, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Sequencing of nucleic acid molecules by mass spectrometry will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFCA-PAI-O-1390489