Sequencing of peptides

C - Chemistry – Metallurgy – 07 – K

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530/7.1

C07K 1/08 (2006.01) C07K 7/10 (1990.01)

Patent

CA 1292840

Abstract of the Disclosure A peptide is sequenced from its carboxyl terminal by coupling with an isothiocyanate having a chemical formula R(X)Si(SCN)y where x=0-3 and y=1-4. R may be an alkyl or an aryl. The coupling reaction is preferably conducted at a temperature range of 50°C-90°C for a period to approximately sixty (60) minutes or less. A weak acid anhydride, preferably a carboxylic acid anhydride, or a weak acid chloride, preferably a carboxylic acid chloride, may be mixed with the isothiocyanate. An organic base catalyst selected from a group consisting of tertiary amines including aromatic amines such as pyridine may be utilized.

519119

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