Sequential hybridization of fungal cell dna and method for...

C - Chemistry – Metallurgy – 12 – Q

Patent

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

C12Q 1/68 (2006.01) C07H 21/04 (2006.01)

Patent

CA 2263992

In order to detect fungal cells in clinical material, fungal DNA is extracted from whole blood, after which the extracted fungal DNA is analysed. On the basis of this analyse a fungal infection can be indicated. For further diagnosis the fungal species is subsequently determined from the extracted fungal DNA. The process for the extraction of fungal DNA from whole blood encompasses the isolation of predominantly intact fungal cells from whole blood and the extraction of DNA from the isolated fungal cells. In order to analyse the fungal DNA at least one segment of said fungal DNA is amplified, whereupon the amplification products are, if required, analysed. 1n order to provide further identification of the fungal species nucleotide sequence segments characteristic of the species of fungus are determined.

Pour détecter des cellules fongiques dans du matériau clinique, on extrait de l'ADN fongique de sang entier et on analyse ensuite cet ADN fongique extrait. Sur la base de cette détection, on peut établir la présence d'une infection fongique. Pour un diagnostic plus précis, on détermine les espèces fongiques à partir de l'ADN fongique extrait. Le procédé d'extraction de l'ADN fongique de sang entier consiste à isoler des cellules fongiques essentiellement intactes du sang entier et à extraire l'ADN des cellules fongiques isolées. Pour analyser l'ADN fongique, on amplifie ensuite au moins un segment de l'ADN fongique à analyser, après quoi les produits d'amplification sont analysés si nécessaire. Pour identifier de façon plus précise l'espèce fongique, on détermine des morceaux de séquences nucléotidiques caractérisant l'espèce fongique.

LandOfFree

Say what you really think

Search LandOfFree.com for Canadian inventors and patents. Rate them and share your experience with other people.

Rating

Sequential hybridization of fungal cell dna and method for... does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Sequential hybridization of fungal cell dna and method for..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Sequential hybridization of fungal cell dna and method for... will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFCA-PAI-O-1703100

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.