C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 5/06 (2006.01) A61K 39/12 (2006.01) C12N 7/00 (2006.01)
Patent
CA 2201585
The invention described herein provides a method for selecting a novel African Green monkey kidney (AGMK) cell substrate, its cultivation and serial passage and its subsequent characterization. The invention further provides a method for the use of the cell substrate in the isolation, growth and serial passage of a large number of viruses, particularly rotaviruses, enteroviruses, respiratory viruses and hepatitis A virus. A method for the utilization of the AGMK cell substrate for the production of live and killed virus vaccines is also provided.
L'invention porte sur un procédé qui permet de sélectionner un nouveau substrat de cellules rénales du singe vert africain (AGMK), sa culture, son passage en série et sa caractérisation ultérieure. L'invention concerne aussi un procédé permettant d'utiliser le substrat de cellules pour l'isolement, la croissance et le passage en série d'un grand nombre de virus, notamment les rotavirus, les entérovirus, les virus des voies respiratoires et les virus de l'hépatite A. L'invention comprend également un procédé permettant d'utiliser le substrat de cellules AGMK pour produire des vaccins à virus actif et des vaccins à virus inactivé.
Chanock Robert M.
Kapikian Albert Z.
Potash Louis
Purcell Robert H.
Dyncorp
National Institutes Of Health
Osler Hoskin & Harcourt Llp
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