C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
73/55, 150/8.5
C12Q 1/14 (2006.01) G01N 21/27 (2006.01)
Patent
CA 1221299
APPLICANT: STATENS BAKTERIOLOGISKA LABORATORIUM TITLE: SEROLOGIC DIAGNOSTICS OF B- STREPTOCOCCAL INFECTIONS WITH THE USE OF AUTOMATIC READING WITH PHOTOMETER FOR MICROTITRATION PLATES ABSTRACT OF THE DISCLOSURE . After infection with B-haemolyzing streptococci, antibodies in the patient serum is detected by neutralizing the serum with streptococcal DNase B on microtitration plates. The reaction is read off either by coloring the substrate with methyl green, illuminating it with monochro- matic light and reading it by means of a photometer provided with a 640 nm interference filter, or the reading is per- formed by means of UV-light having a wavelength of 260 or 254 nm. Colorless DNA is used as a substrate, and the microtitration plates are made of UV-transparent plastics. By connecting a microcomputer to the photometer device, automatically processed analysis results can be obtained. Readings can be performed on one sample from a series of dilutions, or on one and the same sample at specific time intervals.
460643
Ogilvy Renault
Statens Bakteriologiska Laboratorium
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