Serum bactericidal assay for n. meningitidis specific antisera

Details Serum bactericidal assay for n. meningitidis specific antisera Serum bactericidal assay for n. meningitidis specific antisera

G - Physics

01

N

G01N 33/569 (2006.01) C12N 1/22 (2006.01)

Patent

CA 2621578

The present invention relates to the field of Serum Bactericidal Activity (SBA) assays for Gram negative bacteria, in particular N. meningitidis. The SBA assay is the most important method for measuring functional activity of serum antibodies against meningococcus. In order to determine whether a subject or a population is seropositive against invasive meningococcus the SBA test should ideally be both sensitive and specific. The inventors have found the standard N. meningitidis serotype A and W SBAs can be significantly improved in this regard.

La présente invention concerne le domaine des essais d'activité bactéricide sérique (SBA) pour des bactéries Gram négatif, en particulier N. meningitidis. L'essai SBA est le procédé le plus important pour mesurer l'activité fonctionnelle d'anticorps sériques contre les méningocoques. Afin de déterminer si un sujet ou une population est séropositive contre des méningocoques invasifs l'essai SBA doit idéalement être à la fois sensible et spécifique. Les inventeurs ont découvert que les essais SBA des sérotypes standard A et W de N. meningitidis peuvent être significativement améliorés à cet égard.

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