Serum-free cell culture media

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 5/00 (2006.01)

Patent

CA 2273082

Cell culture media containing a sterol such as cholesterol stabilised by surfactant rather than serum products of phospholipid micelles and containing soluble carboxylic acids as fatty acid precursors to satisfy lipid requirements are further enhanced by the presence of alcohols promoting cell growth.

L'invention a pour objet des milieux de cultures cellulaires contenant un stérol, par exemple, un cholestérol stabilisé par un tensioactif, au lieu de produits sériques ou de micelles de phospholides, et contenant également des acides carboxyliques solubles tels que des précurseurs d'acides gras pour satisfaire les exigences des lipides. Ces milieux peuvent être améliorés par la présence d'alcools favorisant la croissance cellulaire.

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