Serum-free growth medium and use thereof

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

195/40, 150/16.1

C12N 5/00 (2006.01) C12N 5/18 (2006.01) C12Q 1/02 (2006.01)

Patent

CA 1321961

- 1 - Abstract The invention provides a serum-free growth medium comprising an iron-chelate, aurin-tricarboxylic acid and optionally alkali-metal-EDTA and trace elements together with growth factors. The iron-chelate preferably comprises a mixture of Fe-EDTA and citric acid. The growth medium may be used for culturing cells and also for quality testing of other growth media, optionally together with a novel hybridoma cell line from p3U1 tumor cells and B-lymphocytes, which hybridoma also forms part of the invention.

556106

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