Serum-free stable transfection and production of recombinant...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/85 (2006.01) C12N 5/10 (2006.01) C12N 15/67 (2006.01) C12N 15/79 (2006.01) C12P 21/02 (2006.01)

Patent

CA 2611708

The present invention relates to an improved method for the serum-free production of an immortalized human cell line stably transfected under serum- free conditions with a specific vector carrying the gene coding for the protein of interest. Furthermore the invention relates to a production cell line obtained by said method, a production method for said protein of interest utilizing said production cell line, and the specific vector carrying the gene of interest itself.

La présente invention concerne un procédé amélioré permettant la production sans sérum d'une lignée cellulaire humaine immortalisée transfectée de manière stable dans des conditions sans sérum avec un vecteur spécifique portant le gène qui code pour la protéine d'intérêt. L'invention se rapporte en outre à une lignée cellulaire de production obtenue selon le procédé précité, à un procédé de production de la protéine d'intérêt précitée au moyen de ladite lignée cellulaire de production, et au vecteur spécifique portant le gène d'intérêt lui-même.

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