Sham-sensitive terminal oxidase gene from xylose-fermenting...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/53 (2006.01) C12N 1/16 (2006.01) C12N 1/19 (2006.01) C12N 9/02 (2006.01) C12N 15/81 (2006.01) C12P 7/10 (2006.01)

Patent

CA 2406258

Disclosed is a SHAM-sensitive terminal oxidase (STO) gene from the xylose- fermenting yeast Pichia stipitis. Also disclosed is a mutant of Pichia stipitis in which the STO gene natively present in the wild-type yeast was disrupted. Mutants of Pichia stipitis having reduced expression of PsSTO were found to exhibit enhanced fermentation of xylose to ethanol.

Cette invention concerne une oxydase terminale sensible (STO) à SHAM (salicyl hydroxymate) tirée de la levure <i>Pichia stipitis</i> de fermentation du xylose. L'invention porte également sur un mutant de <i>Pichia stipitis </i>dans lequel le gène STO présent à l'état natif dans la levure de type sAuvage a été interrompu. On a constaté que des mutants de <i>Pichia stipitis</i> avec expression réduite de PsSTO se caractérisaient par une fermentation accrue du xylose en éthanol.

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