Short enzyme donor fragment

Details Short enzyme donor fragment Short enzyme donor fragment

C - Chemistry, Metallurgy

12

N

C12N 9/38 (2006.01) C07K 19/00 (2006.01) C12N 15/56 (2006.01) C12Q 1/34 (2006.01) C12Q 1/68 (2006.01) G01N 33/573 (2006.01)

Patent

CA 2484499

Short enzyme donor fragments of .beta.-galactosidase are provided of not more than 40 amino acids, where the short fragments are used as a label and may be substituted with a wide variety of organic compounds, particularly polypeptides having independent functional activity. The enzyme donor finds use in competitive and non-competitive assays, monitoring intracellular events, or other processes where a sensitive non-interfering label is desire.

L'invention concerne des fragments courts d'enzyme donneur de .beta.-galactosidase ne contenant pas plus de 40 acides aminés, ces fragments courts étant utilisés comme étiquette et pouvant être substitués avec une grande variété de composés organiques, particulièrement des polypeptides possédant une activité fonctionnelle indépendante. L'enzyme donneur est utilisée dans des tests compétitifs et non compétitifs, dans le suivi d'évènements intracellulaires, ou d'autres procédés dans lesquels on a besoin d'une étiquette sensible n'interférant pas.

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