Short-term anti-cd3 stimulation of lymphocytes to increase...

C - Chemistry – Metallurgy – 12 – N

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C12N 5/00 (2006.01) A61K 35/18 (2006.01) A61K 38/20 (2006.01) C12N 5/06 (2006.01) A61K 35/12 (2006.01)

Patent

CA 2111564

2111564 9300918 PCTABS00019 The present invention is directed to a method of increasing the in vivo immune response of lymphocytes by stimulating the lymphocytes in vitro in the presence of an anti-CD3 monoclonal antibody for less than about 24 hours to form stimulated lymphocytes; infusing the stimulated lymphocytes into a tumor-bearing mammal; and administering IL-2 to the mammal. As a result of this method, the anti-CD3 stimulated lymphocytes display enhanced immunotherapeutic, e.g., cytotoxicity or lymphokine production, in vivo) as represented by a decrease in the number of tumors by at least about 20 %.

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