Shotgun scanning, a combinatorial method for mapping...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/10 (2006.01) C07K 16/32 (2006.01) C12N 15/62 (2006.01) C12N 15/63 (2006.01)

Patent

CA 2393869

A combinatorial method that uses statistics and DNA sequence analysis rapidly assesses the functional and structural importance of individual protein side chains to binding interactions. This general method, termed "shotgun scanning", enables the rapid mapping of functional protein and peptide epitopes and is suitable for high throughput proteomics.

La présente invention concerne un procédé combinatoire qui fait intervenir les statistiques et l'analyse de séquence d'ADN, permettant d'évaluer rapidement l'importance fonctionnelle et structurelle de chaînes latérales de protéines individuelles vis-à-vis des interactions de liaison. Ce procédé, appelé "balayage aveugle", permet une représentation rapide d'épitopes de protéines et peptides fonctionnels, et convient dans le cadre d'une protéomique efficace.

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