Shuffling of codon altered genes

Details Shuffling of codon altered genes Shuffling of codon altered genes

C - Chemistry, Metallurgy

12

N

C12N 15/10 (2006.01) A61K 39/21 (2006.01) B01J 19/00 (2006.01) C07B 61/00 (2006.01) C07K 14/16 (2006.01) C07K 14/505 (2006.01) C07K 14/535 (2006.01) C12N 5/10 (2006.01) C12N 7/04 (2006.01) C12N 9/16 (2006.01) C12N 15/11 (2006.01) C12N 15/12 (2006.01) C12N 15/49 (2006.01) C12N 15/55 (2006.01) G06F 19/00 (2006.01) A61K 39/00 (2006.01)

Patent

CA 2331335

Methods of recombining codon-altered libraries of nucleic acids are provided. The nucleic acids can include conservative or non-conservative modifications of coding sequences, in addition to codon alterations, as compared with wild- type sequences. In addition to making new proteins, methods of generation vectors with reduced rates of reversion to wild-type and attenuated viruses are also provided.

La présente invention concerne des techniques de recombinaison de banques, modifiées par codon, d'acides nucléiques. En plus des modifications par codon, ces acides nucléiques peuvent inclure des modifications conservatrices ou non conservatrices des séquences de codage, par opposition à des séquences de type sauvage. L'invention concerne l'obtention, outre de nouvelles protéines, de vecteurs caractérisés par des taux réduits de retransformation en virus de type sauvage et atténué.

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