Shuttle vectors for the introduction of dna into...

Details Shuttle vectors for the introduction of dna into... Shuttle vectors for the introduction of dna into...

C - Chemistry, Metallurgy

12

N

C12N 15/74 (2006.01) A61K 39/04 (2006.01) C12N 1/21 (2006.01) C12N 9/02 (2006.01) C12N 9/88 (2006.01) C12N 15/52 (2006.01) C12N 15/53 (2006.01) C12N 15/60 (2006.01)

Patent

CA 2190861

Shuttle vectors for the introduction of DNA into mycobacteria comprising at least one functional replication origin in said mycobacteria, another functional replication origin in other bacteria, an enzymatic cleavage site providing for the insertion of DNA coding for a protein susceptible of being expressed in the mycobacteria, characterized in that the vectors also carry a gene which makes the mycobacteria resistant to a compound containing a heavy metal.

Vecteurs navettes pour l'introduction d'ADN dans des mycobactéries comprenant au moins une origine de réplication fonctionnelle dans lesdites mycobactéries, une autre origine de réplication fonctionnelle dans d'autres bactéries, un site de coupure enzymatique permettant l'insertion d'ADN codant pour une protéine susceptible d'être exprimée dans les mycobactéries, caractérisés en ce qu'ils portent en plus un gène conférant auxdites mycobactéries la résistance à un composé contenant un métal lourd.

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