C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/68 (2006.01)
Patent
CA 2515734
It is intended to provide a signal amplification method for detecting an expressed gene by which detection can be made at a low cost by a simple procedure within a short time without resort to any expensive enzymes and which enables detection corresponding to the original RNA length or expression amount because of using neither linear amplification nor PCR. Using a reverse transcription reaction and a self assembly reaction of forming a self-assembly due to the self assembly of oligonucleotide probes, the sensitivity of a DNA chip of detecting an expressed gene is elevated.
L'invention concerne un procédé d'amplification de signaux destiné à détecter un gène exprimé qui permet de détecter un gène exprimé à peu de frais au moyen d'une procédure simple, dans un bref délai et sans recourir aux enzymes coûteuses; il permet d'effectuer une détection correspondant à la longueur ou à la quantité d'expression d'ADN d'origine car il n'utilise ni l'amplification linéaire ni le PCR. L'utilisation d'une réaction de transcription inverse et d'une réaction d'auto-assemblage pour former un assemblage autonome grâce aux activités des sondes oligonucléotidiques permet d'augmenter la sensibilité d'une puce ADN dans la détection d'un gène exprimé.
Fujikawa Toshihiko
Usui Mitsugu
Eisai Co. Ltd.
Eisai R&d Management Co. Ltd.
Norton Rose Or S.e.n.c.r.l. S.r.l./llp
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