Signal amplification using a synthetic zymogen

Details Signal amplification using a synthetic zymogen Signal amplification using a synthetic zymogen

C - Chemistry, Metallurgy

12

Q

C12Q 1/04 (2006.01) G01N 33/542 (2006.01) G01N 33/58 (2006.01) G01N 33/68 (2006.01)

Patent

CA 2537529

Described herein are zymogens, methods of use for zymogens, and devices that incorporate zymogens. The zymogens include a substrate and an enzyme. The substrate can inhibit the enzyme and is a target for a protein produced by a microorganism. When the substrate is modified by a protein produced by a microorganism, the enzyme is activated. The zymogens can be used to amplify detection assays.

L'invention décrit des proenzymes, des procédés d'utilisation de ces proenzymes ainsi que des dispositifs qui comprennent ces proenzymes. Ces proenzymes contiennent un substrat et une enzyme. Le substrat, qui peut inhiber l'enzyme, représente une cible pour une protéine produite par un micro-organisme. Lorsque le substrat est modifié par une protéine produite par un micro-organisme, l'enzyme est activée. Par ailleurs, ces proenzymes peut être utilisées pour amplifier les méthodes de détection.

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