Signal system and elements used therein

Details Signal system and elements used therein Signal system and elements used therein

C - Chemistry, Metallurgy

12

N

C12N 15/62 (2006.01) C07K 14/435 (2006.01) C07K 19/00 (2006.01) C12N 9/02 (2006.01) C12N 9/90 (2006.01) C12N 15/12 (2006.01) C12N 15/53 (2006.01) C12N 15/61 (2006.01) C12Q 1/66 (2006.01)

Patent

CA 2464942

A method for measuring intracellular ATP and/or gene expression, which method comprises transforming a cell with a construct which encodes a luciferase and a luciferin recycling protein, introducing luciferin into said cell, and monitoring biolominescent signals from said cell. Novel luciferin recycling proteins are also described and these, together with their use in the production of optically active enzyme substrates are claimed.

La présente invention concerne une méthode permettant de mesurer l'expression intracellulaire de l'ATP et/ou l'expression génétique. Ladite méthode consiste à transformer une cellule à l'aide d'un gène hybride qui code une luciférase et une protéine de recyclage de luciférine, introduire la luciférine dans ladite cellule, et surveiller les signaux biolominescents issus de ladite cellule. L'invention concerne également de nouvelles protéines de recyclage de luciférine ainsi que leur utilisation dans la production de substrats d'enzymes optiquement actifs.

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