Simplified solid-phase immunobead assay for detection of...

Details Simplified solid-phase immunobead assay for detection of... Simplified solid-phase immunobead assay for detection of...

G - Physics

01

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G01N 33/546 (2006.01) G01N 33/53 (2006.01) G01N 33/569 (2006.01) G01N 33/577 (2006.01)

Patent

CA 2080925

2080925 9116631 PCTABS00008 The development of a highly-simplified solid-phase colored latex immunobead assay for the detection of ciguatoxin and related polyethers. This procedure was compared with a previously-reported stick enzyme immunoassay. Chi square analysis of two separate experiments 153 and 283 fish of various species gave X2 values of p < 0.001 and < 0.005, respectively. Agreement between the two procedures with 26 fish implicated in ciguatera poisoning was 100 %. A preliminary assessment in the field showed encouraging results. The procedure appears to be simple and applicable to field use. Furthermore, this procedure should be applicable for other antibody-antigen detections, especially low dalton determinants.

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