Simulated activity of protein a displayed by ligands...

B - Operations – Transporting – 01 – J

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B01J 20/32 (2006.01) C07K 1/22 (2006.01) C07K 16/06 (2006.01)

Patent

CA 2407276

A method and compound for the purification of proteins including the attachment of a non-peptidic, small compound which simulates the affinity of Protein A for immunoglobulins to a support matrix. Once attached on the support matrix, the resulting monochloro-triazine derivative is reacted with an excess of an amino compound at a higher temperature to achieve high levels of substitution. The resulting support matrix with ligand is useful in the affinity separations of antibodies. Further, a mercapto heterocyclic system ligand may be attached to the super matrix and useful in affinity separations of antibodies.

L'invention se rapporte à un procédé et à un composé permettant la purification de protéines. Ledit procédé consiste notamment en la fixation sur une matrice support d'un petit composé non peptidique qui simule l'affinité de la protéine A pour les immunoglobulines. Une fois fixée à la matrice support, le dérivé résultant monochloro-triazine est amené à réagir avec un composé amino en excès, à une température supérieure, de manière à produire de hauts niveaux de substitution. La matrice support résultante munie du ligand est utile dans les séparations par affinité d'anticorps. En outre, un ligand de système hétérocyclique mercapto peut être fixé à la super matrice et s'avérer utile dans les séparations par affinité d'anticorps.

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