Simultaneous assay for cholesterol and triglyceride

Details Simultaneous assay for cholesterol and triglyceride Simultaneous assay for cholesterol and triglyceride

C - Chemistry, Metallurgy

12

Q

150/11, 150/26,

C12Q 1/61 (2006.01) C12Q 1/60 (2006.01)

Patent

CA 1323554

ABSTRACT A method for the simultaneous determination of cholesterol and triglyceride substrates with a single reagent system. The reagent system is reacted with the specimen such that each of the substrates react with their respective reactant simultaneously. The change in absorbance or fluorescence of the resulting reaction mixture is measured at a plurality of wavelengths which are characteristic for each of the substrates to be determined. The amount of cholesterol and triglyceride substrates can be determined by either an endpoint or reaction rate measurement. The reagent system comprises an enzyme having cholesterol esterase activity, a chromogenic oxygen acceptor, microperoxidase, and cholesterol oxidase for determination of cholesterol; and lipase, ATP, PEP, glycerol kinase, pyruvate kinase, LDH and NAD(P)H or analogs thereof for determination of triglyceride.

578065

Affiliated with

Also associated with

Rating

Simultaneous assay for cholesterol and triglyceride does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Simultaneous assay for cholesterol and triglyceride, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Simultaneous assay for cholesterol and triglyceride will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFCA-PAI-O-1316815