Simultaneous calibration heterogeneous immunoassay

Details Simultaneous calibration heterogeneous immunoassay Simultaneous calibration heterogeneous immunoassay

G - Physics

01

N

167/43, 167/45

G01N 33/532 (2006.01) G01N 33/542 (2006.01) G01N 33/543 (2006.01)

Patent

CA 1193193

ABSTRACT OF THE DISCLOSURE An assay method and compositions are provided for determining the presence of an analyte in a sample. The analyte is a member of an immunological pair (mip) compris- ing ligand and receptor. By providing a first measurement surface capable of specifically binding a labelled reagent in an amount depending upon the presence of analyte in the sample and a second calibration surface capable of binding a second labeled reagent in a manner unaffected by the presence of analyte in the sample, calibration of individual tests can be accomplished simultaneously with the performance of the test itself. A signal producing system includes an enzyme, a catalyst usually bonded to a mip which defines the first labeled reagent for binding to the measurement surface and the same catalyst conjugated to a ligand capable of binding to the calibration surface. Preferably, both labeled reagents have the same composition and the calibration surface includes anti-(first catalyst).

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