Simultaneous dual analyte assay

Details Simultaneous dual analyte assay Simultaneous dual analyte assay

G - Physics

01

N

G01N 33/535 (2006.01) A61K 39/395 (2006.01) G01N 33/543 (2006.01) G01N 33/74 (2006.01)

Patent

CA 2049036

2049036 9011521 PCTABS00002 A simultaneous dual analyte assay for determining the fertile period of the human menstrual cycle. The assay utilizes a capture reaction component consisting of P-3-G immobilized on a microporous membrane, a blocking reaction component consisting of anti E1-3-G antibody, a labelled reaction component consisting of gold particle labelled anti E1-3-G antibody, and an ambifunctional reaction component consisting of a hybrid immunoreactive substance having an anti P-3-G antibody binding site and a plurality of E1-3-G determinant binding sites. An aqueous sample containing P-3-G and E1-3-G is contacted with the components and the assay is calibrated to provide a positive assay result only when the concentration of P-3-G in the sample is less than a predetermined concentration and the concentration of E1-3-G in the sample is more than a preselected concentration whereby a visually inspectable quantity of an immunocomposite is produced that consists of both the P-3-G immobilized on the microporous membrane and the gold labelled anti E1-3-G antibody.

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