G - Physics – 01 – N
Patent
G - Physics
01
N
167/44
G01N 33/53 (2006.01) G01N 33/543 (2006.01) G01N 33/545 (2006.01) G01N 33/569 (2006.01)
Patent
CA 1303494
ABSTRACT OF THE DISCLOSURE A solid-phase immunoassay is provided for determination of members of an immunological pair of such antigen and/or antibody of a single binding pair in human physiological fluid, wherein a single immunoassay enables simultaneous detection of antigen and/or antibody of a single binding pair in a test sample which may have circulating antigen and/or antibody. The immunoassay is characterized by the addition of an amount of antigen or "spike" of the binding pair to the test sample prior to incuba- tion of the test sample in the presence of a solid-phase ab- sorbed antibody of the binding pair. The test sample preferably also is subjected to a lysing reagent prior to the incubation for uniformly dispensing antigens which may be present in the test sample.
566855
Allman David M.
Forman Meryl A.
Hofheinz David E.
Kortright Kenneth H.
Lee Song Y.
Allman David M.
Coulter Corporation
Forman Meryl A.
Hofheinz David E.
Kortright Kenneth H.
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