Single cell microinjection using flexible and compliant...

Details Single cell microinjection using flexible and compliant... Single cell microinjection using flexible and compliant...

C - Chemistry, Metallurgy

12

M

C12M 3/00 (2006.01) B81B 3/00 (2006.01) C12M 1/42 (2006.01) C12N 15/87 (2006.01) C12N 15/89 (2006.01)

Patent

CA 2685615

A microinjection device for injecting a target cell with a reagent. The microinjection device includes a flexible substrate; a target supply channel formed in the flexible substrate for receiving the target cell; a reagent supply channel formed in the flexible substrate for receiving the reagent; a suction capillary mounted within a suction channel formed in the flexible substrate, the suction capillary providing suction to the target supply channel for immobilizing the target cell within the target supply channel; an injection needle mounted within a needle channel formed in the flexible substrate, the injection needle being movable between injected and un-injected positions by deforming at least a part of the flexible substrate; and a plurality of electrodes embedded in the flexible substrate, the plurality of electrodes creating a voltage potential across the injection needle to move the reagent into the target when the injection needle is in the injected position.

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