Single-chain antibody with cleavable linker

Details Single-chain antibody with cleavable linker Single-chain antibody with cleavable linker

C - Chemistry, Metallurgy

07

K

C07K 5/00 (2006.01) A61K 39/395 (2006.01) C07K 16/00 (2006.01) C12N 1/15 (2006.01) C12N 1/19 (2006.01) C12N 15/63 (2006.01) C12P 21/00 (2006.01)

Patent

CA 2604786

Disclosed are antibodies and methods for making antibodies with desired glycosylation and efficient production. Host cells transformed with a nucleic acid encoding a fusion protein comprising a signal sequence, light and heavy immunoglobulin chains, each comprising a variable region and a constant region and separated by a spacer peptide comprising at least one proteolytic cleavage site are cultured to express the nucleic acids and are cleaved by appropriate proteases to produce antibodies.

L'invention concerne des anticorps et des procédés de fabrication d'anticorps présentant une glycosylation désirée et une production efficace. Des cellules hôtes transformées au moyen d'un acide nucléique codant une protéine hybride comprenant une séquence-signal, des chaînes d'immunoglobuline légères et lourdes, chacune comprenant une région variable et une région constante, séparées par un peptide espaceur comprenant au moins un site de clivage protéolytique, sont cultivées pour exprimer les acides nucléiques et clivées au moyen de protéases appropriées afin de produire des anticorps.

Affiliated with

Also associated with

Rating

Single-chain antibody with cleavable linker does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Single-chain antibody with cleavable linker, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Single-chain antibody with cleavable linker will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFCA-PAI-O-1729628