Single channel, single dilution detection method

Details Single channel, single dilution detection method Single channel, single dilution detection method

G - Physics

01

N

G01N 33/50 (2006.01) G01N 15/14 (2006.01)

Patent

CA 2367780

The invention provides a single dilution method and system for identifying, analyzing and quantifying the cellular components of whole blood using a single channel of an automated hematology analyzer utilizing flow cytometry and the detection of the light scattered and absorbed by each cell. The method involves the use of an organic dye in the reagent solution for staining the nucleic acid of reticulocytes, including reticulated platelets, and white blood cells in the sample. The single channel method is particularly useful for determining white blood cell counts and assessing parameters of a whole blood sample, for blood samples from both human and non-human mammals. The apparatus for performing the method provides an economical, streamlined and space-saving analyzer for carrying out complete blood cell analysis on a mammalian whole blood sample.

L'invention concerne un procédé et un système de dilution unique permettant d'identifier, d'analyser et de quantifier les composants cellulaires du sang entier au moyen d'un canal unique, d'un analyseur hématologique par cytométrie de flux ; et par détection de la lumière diffusée et absorbée par chaque cellule. Le procédé consiste à utiliser un colorant organique dans la solution de réactif pour colorer l'acide nucléique des réticulocytes, y compris les plaquettes réticulées et les globules blancs dans l'échantillon. Le procédé en canal unique selon la présente invention est particulièrement utile pour déterminer le nombre de globules blancs et évaluer les paramètres d'un échantillon de sang entier provenant de mammaliens humains et non humains. L'appareil de mise en oeuvre du procédé constitue un analyseur bon marché, rationalisé et non encombrant permettant d'analyser complètement une cellule sanguine dans un échantillon de sang entier de mammalien.

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