Single step heterogenous assay

G - Physics – 01 – N

Patent

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167/44

G01N 33/543 (2006.01) G01N 33/535 (2006.01)

Patent

CA 1253070

ABSTRACT OF THE DISCLOSURE Methods and devices are provided involving heterogeneous enzyme assays. A simplified protocol for the assays results from incorporating enzyme substrate on a bibulous support to which is conjugated a member of a specific binding pair. Upon introduction of the support into the assay medium, the substrate diffuses into the medium, eliminating a separate development step for the assay.

479634

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