C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
150/14, 150/15.3
C12Q 1/56 (2006.01) C12Q 1/37 (2006.01)
Patent
CA 1254119
ABSTRACT OF THE DISCLOSURE The invention provides a single step assay method for a protease inhibitor or its activator and a test device, a method for its preparation and its use. Whereas current procedures require sequen- tial addition of reagents and incubation with each addition to produce the same results, the method of the present invention involves essentially simul- taneous contact of the composition components and an aqueous test sample. The composition includes a protease capable of being inhibited by the inhibi- tor of interest and a substrate capable of being cleaved by the protease to produce a detectable response either directly or after reaction with an additionally provided coupling component. In a preferred embodiment the concentration of anti- thrombin III in a diluted plasma sample can be determined with a test device which is prepared by incorporation of a carrier matrix with thrombin and with a chromogenic substrate so that they remain in substantially unreacted form prior to contact with the test sample. Results are obtained by monitoring the observable response continuously for two minutes or less. The invention can also be used for the determination of an activator of a protease inhibitor.
479651
Miles Laboratories Inc.
Osler Hoskin & Harcourt Llp
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