Single vial technique for radio-labeling protein

Details Single vial technique for radio-labeling protein Single vial technique for radio-labeling protein

G - Physics

01

N

167/40, 530/15.0

G01N 33/534 (2006.01) G01N 33/60 (2006.01)

Patent

CA 1318614

ABSTRACT Labeling protein with a radioisotope by placing a reagent which catalyzes the coupling of the radioisotope to protein into a sealable reaction vessel, in which the reagents can be intro- duced and withdrawn, sealing the vessel; introducing into the vessel the radioisotope and a buffer solution of the protein to be labeled; incubating the resulting reaction mixture under conditions which permit the labeling of the protein; introducing a resin which adsorbs the radioisotope which is not bound to protein into the vial; withdrawing the resin and the reaction mixture; and separating the resin and the protein. This method is simple, efficient, and reproducible and it minimized the safety hazards to persons performing the radio- labeling.

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