C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 1/21 (2006.01) C12N 15/52 (2006.01) C12N 15/74 (2006.01) C12P 7/06 (2006.01)
Patent
CA 2289091
A microorganism of the genus Zymomonas containing exogenous genes encoding xylose isomerase, xylulokinase, L-arabinose isomerase, L-ribulokinase, L-ribulose-5-phosphate 4-epimerase, transaldolase and transketolase and further comprising at least one promotor recognized by Zymomonas which regulates the expression of at least one of the genes, wherein the microorganism is capable of growing on arabinose and xylose as carbon sources and fermenting the arabinose and xylose to ethanol in about 75 % theoretical yield and, wherein the microorganism without the genes is incapable of growing on or fermenting the arabinose and xylose to ethanol.
Dans la présente invention, on divulgue un micro-organisme du genre Zymomonas contenant des gènes exogènes codant la xylose-isomérase, la xylulokinase, la L-arabinose-isomérase, la L-ribulokinase, la L-ribulose-5-phosphate-4-épimérase, la transaldolase et la transcétolase, et comportant également au moins un promoteur reconnu par Zymomonas qui régule l'expression d'au moins un des gènes. Ce micro-organisme est apte à une croissance sur l'arabinose et le xylose tenant lieu de sources de carbone et permet la fermentation de l'arabinose et du xylose pour produire de l'éthanol avec un rendement théorique d'environ 75 %. Ce micro-organisme sans les gènes ne permet pas la culture sur l'arabinose et le xylose ni leur fermentation pour donner de l'éthanol.
Chou Yat-Chen
Finkelstein Mark
Picataggio Stephen K.
Zhang Min
Alliance For Sustainable Energy Llc
Finlayson & Singlehurst
Midwest Research Institute
LandOfFree
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