Site-specific incorporation of amino acids into molecules

Details Site-specific incorporation of amino acids into molecules Site-specific incorporation of amino acids into molecules

C - Chemistry, Metallurgy

12

N

C12N 15/09 (2006.01)

Patent

CA 2644474

The invention provides certain embodiments relating to methods and compositions for incorporating non-natural amino acids into a polypeptid or protein by utilizing a mutant or modified aminoacyl-tRNA synthetase to charge the non-natural amino acid to a the corresponding tRNA. In certain embodiments, the tRNA is also modified such that the complex forms strict Watson-Crick base-pairing with a codon that normally forms wobble base- pairing with unmodified tRNA/aminoacyl-tRNA synthetase pairs.

Certains modes de réalisation de l'invention concernent des procédés et des compositions permettant d'incorporer des acides aminés artificiels dans un polypeptide ou une protéine au moyen d'une aminoacyl-ARNt synthétase mutante ou modifiée pour charger l'acide aminé artificiel sur un ARNt correspondant. Dans certains modes de réalisation, l'ARNt est également modifié de façon que le complexe forme un appariement de bases Watson-Crick strict avec un codon formant normalement un appariement de bases Wobble avec des paires ARNt/aminoacyl-ARNt synthétase non modifiées.

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