Site-specific incorporation of redox active amino acids into...

Details Site-specific incorporation of redox active amino acids into... Site-specific incorporation of redox active amino acids into...

C - Chemistry, Metallurgy

12

N

C12N 15/52 (2006.01) C12N 1/21 (2006.01) C12N 9/10 (2006.01) C12N 15/70 (2006.01) C12N 15/74 (2006.01) C12P 21/00 (2006.01) C12Q 1/48 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2541166

Compositions and methods of producing components of protein biosynthetic machinery that include orthogonal tRNAs, orthogonal aminoacyl-tRNA synthetases, and orthogonal pairs of tRNAs/synthetases, which incorporate redox active amino acids into proteins are provided. Methods for identifying these orthogonal pairs are also provided along with methods of producing proteins with redox active amino acids using these orthogonal pairs.

L'invention concerne des compositions et des méthodes pour produire des constituants d'une construction biosynthétique protéinique comprenant des ARNt orthogonaux, des synthétases d'ARNt aminoacyle orthogonaux et des paires orthogonales d'ARNt/synthétases, intégrant des acides aminés actifs pour une oxydoréduction dans des protéines. L'invention concerne des méthodes pour identifier ces paires orthogonales ainsi que des méthodes pour produire des protéines au moyen d'acides aminés actifs pour une oxydoréduction, lesquelles méthodes faisant appel à ces paires orthogonales.

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