Siv-based packaging-defficient vectors

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/86 (2006.01) A61K 48/00 (2006.01) C12N 7/00 (2006.01) C12N 15/11 (2006.01) C12N 15/867 (2006.01)

Patent

CA 2376790

A simian Immunodeficiency Virus (SIV) genome having a mutation within the packaging signal such that viral RNA is not packaged within an SIV capsid is described. A viral vector comprises an SIV packaging signal and a heterologous gene capable of being expressed in the vector. The packaging defective SIV genome and viral vector may be co-transfected into a host cell to produce SIV virus particles capable of expressing a heterologous gene.

La présente invention concerne un génome du virus de l'immunodéficience simienne (SIV) présentant une mutation dans le signal d'encapsidation de façon que l'ARN viral n'est pas encapsidé dans un capside du SIV. Le vecteur viral de l'invention comprend un signal d'encapsidation SIV et un gène hétérologue capable d'être exprimé dans le vecteur. Le génome du SIV à encapsidation déficiente et le vecteur viral peuvent être co-transfectés dans une cellule hôte pour produire des particules de virus SIV capables d'exprimer un gène hétérologue.

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