Skin equivalent preparation process and said skin equivalent

C - Chemistry – Metallurgy – 12 – N

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C12N 5/08 (2006.01) A61F 2/10 (2006.01) A61L 27/00 (2006.01) A61L 27/24 (2006.01) A61L 27/60 (2006.01) C12N 5/06 (2006.01) A61F 2/00 (2006.01) A61K 35/12 (2006.01)

Patent

CA 1296260

ABREGE PROCEDE D'OBTENTION D'UN EQUIVALENT DE PEAU ET EQUIVALENT DE PEAU CORRESPONDANT. L'invention concerne un procédé d'obtention d'un équivalent de peau et l'équivalent de peau correspon- dant (27). Selon ce procédé, on prépare un équivalent de derme (24) en mélangeant des cellules contractiles, un milieu nutritif et du collagène pour former un gel qui se contracte ; on utilise cet équivalent de derme (24) comme substrat pour un équivalent d'épiderme (26) obtenu par ensemencement avec les kératinocytes d'un explant animal ou humain. Selon l'invention t on ensemence le subs- trat (24) au moyen d'au moins un tronçon de follicule pileux (22) comportant au moins partiellement sa gaine cellulaire, ledit tronçon étant implanté sensiblement perpendiculaire- ment à la surface libre du substrat. Figure à publier : figure 4

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