C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 5/071 (2010.01) A61K 9/12 (2006.01) A61K 35/12 (2006.01) A61K 35/36 (2006.01) A61P 17/00 (2006.01) C12M 3/00 (2006.01)
Patent
CA 2533422
A cell culture medium and system are provided which eliminate or at least reduce the requirement for exogenous components such as serum and feeder cells. The cell culture medium comprises an IGF and vitronectin or fibronectin and, optionally an IGFBP, and is particularly suitable for propagating keratinocytes for subsequent use in skin growth and regeneration. This invention also relates to compositions and methods for skin growth and regenerationin situ, which utilize aerosol delivery of cultured keratinocytes.
L'invention porte sur un milieu de culture de cellules et un système éliminant ou au moins réduisant le recours à des composants exogènes tels que du sérum ou des cellules nourricières. Ledit milieu, qui comprend de l'IGF et de la vitronectine ou de la fibronectine et facultativement de l'IGFBP, est particulièrement adapté à la propagation des kératinocytes utilisables pour la croissance et la régénération de la peau. L'invention porte également sur des préparations et procédés de croissance et régénération in situ de la peau au moyen d'aérosols de kératinocytes de culture.
Harkin Damien
Leavesley David
Upton Zee
Deeth Williams Wall Llp
Queensland University Of Technology
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