Sm-protein based secretion engineering

Details Sm-protein based secretion engineering Sm-protein based secretion engineering

C - Chemistry, Metallurgy

12

P

C12P 21/02 (2006.01) A61K 38/00 (2006.01) A61K 39/00 (2006.01) A61K 48/00 (2006.01) C12N 15/11 (2006.01) C12N 15/12 (2006.01) C12N 15/63 (2006.01) C12N 1/15 (2006.01) C12N 1/21 (2006.01) C12N 5/10 (2006.01)

Patent

CA 2709645

The present invention concerns the field of cell culture technology. It describes a novel method for enhancing the secretory transport of proteins in eukaryotic cells by heterologous expression of Munc18c, Sly1 or other members of the SM protein family. This method is particularly useful for the generation of optimized host cell systems with enhanced production capacity for the expression and manufacture of recombinant protein products.

La présente invention concerne le domaine de la technologie de la culture cellulaire. Elle décrit un nouveau procédé pour améliorer le transport sécrétoire des protéines dans des cellules eucaryotes par expression hétérologue de Munc18c, Sly1 ou d'autres membres de la famille des protéines SM. Ce procédé est particulièrement utile pour générer des systèmes de cellules hôtes optimisés ayant une capacité de production accrue pour l'expression et la fabrication de produits protéiques recombinés.

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