Solid culture medium and the method of its production

C - Chemistry – Metallurgy – 12 – N

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C12N 1/00 (2006.01) C12N 1/14 (2006.01) C12N 1/20 (2006.01)

Patent

CA 2150720

This solid nutrient medium for cultivating microorganisms contains a copolymer of acrylonitrile and acrylamide and a liquid nutrient substrate in the following percent-by-weight proportions: copolymer of acrylonitrile and acrylamide: 5.0 - 30.0; nutrient substrate: 70.0 - 95Ø The method of obtaining a solid culture medium includes the purification of acrylamide and N,N'-methylene-bis-acrylamide by recrystalization; preparation of the reactive mix composed of acrylonitrile, acrylamide, N,N'-methylene-bis-acrylamide, an acceptable solvent and an initiating system; polymerization over a period sufficient for the production of a copolymer retaining the form; its rinsing and saturation with water until equilbrium state; permeation with the nutrient substrate, where the components have these percent-by- weight proportions: acrylonitrile 5.0 - 50.0; acrylamide 5.0 - 50.0; N,N'-methylene-bis-acrylamide 0.01 - 0.60;initiating system 0.15 - 0.3; acceptable solvent residuum.

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