Solid phase amplification process

Details Solid phase amplification process Solid phase amplification process

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01)

Patent

CA 2122450

An assay and method of use thereof for amplification and detection of target nucleic acids in patient samples, which is rapid, simple and accurate is described. The assay and method utilizes any one of a variety of amplification methods, in combination with primers immobilized onto either the container containing the patient sample, such as a microtiter welt plate, or a dipstick which is readily immersible into the container. Amplification occurs in the presence of both the first, immobilized primer and a second, labelled primer which hybridizes to the target nucleic acid in the opposite direction to the first. Examples demonstrate the effectiveness of the method in detecting a specific species of mycoplasma, as well as the gene characteristic of cystic fibrosis.

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