Solid phase diffusion assay

Details Solid phase diffusion assay Solid phase diffusion assay

G - Physics

01

N

150/14, 167/44,

G01N 33/53 (2006.01) G01N 33/543 (2006.01)

Patent

CA 1268420

ABSTRACT OF THE DISCLOSURE A method is disclosed for the quantitative or qualitative determination of an analyte in a test sample comprising the steps of: (a) binding a first substance to an insoluble porous sheet support said first substance being selected from the group consisting of ligands and receptors which bind said analyte, said support being covered by a layer of water impermeable material having a hole therein; (b) applying an aliquot of said test sample to said insoluble support through said hole; (c) permitting said test sample to diffuse through said insoluble support carrying said first substance; (d) applying a second substance to said insoluble support before, together with, or after application of said analyte, said second substance being selected from the group of ligands and receptors capable of being immobilized by binding to either said first substance or said analyte; and (e) assessing the presence and/or quantity of said immobilized labelled second substance by means of a label attached thereto either before or after said immobilization, said assessment being effected in the case of lateral diffusion by measuring the amount of said diffusion. The label is advantageously colloidal gold or silver when this label is used, the layer of water impermeable material having a hole therein may optionally be omitted. Kits for the qualitative or quantitative determination of analyte in a test sample are also disclosed.

498318

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