G - Physics – 01 – N
Patent
G - Physics
01
N
167/45
G01N 33/50 (2006.01) G01N 33/543 (2006.01)
Patent
CA 1113858
ABSTRACT OF THE DISCLOSURE The specification discloses a method of analysing a liquid biological medium such as human blood in order to detect or identify viruses therein having antigen properties (i.e. virus antigens) or erythrocyte or cell antigens or antibodies. According to the disclosure in order to determine whether a biological medium contains virus antigens or erythrocyte or cell antigens or antibodies, a method of analysis is used involving immunological reactions between cells or particles and a serum and then between the thus treated cells or particles and an antiglobulin, the method being characterised in that the reaction medium containing the cells or particles and the antiglobulin is introduced into a vessel, the wall of which has a point of convergence or apex situated on the axis of symmetry of the vessel, molecules capable of an immunological reaction with the antiglobulin having been fixed on the walls of the vessel, and the reaction medium is subjected to a centrifugal force substantially parallel to the aforementioned axis under con- ditions such that at least the cells showing a positive reaction, i.e. an analytical reaction performed on a sample of the biological medium containing the antigen or antibody to be identified, progressively stick to and remain stuck to the vessel wall by bonding forces of a first kind, i.e. immunological bonding forces involving the antiglobulin, which are added to bonding forces of a second kind, whereas the cells showing a negative reaction at the end of centrifuging are not stuck to the wall except by bonding forces of the second kind, so that the last-mentioned cells are collected at the apex of the vessel by applying a centrifugal force sufficiently strong to destroy the bonding forces of the second kind (i.e. connecting a negative cell to the vessel wall) but not sufficient to destroy the sum of the bonding forces of the first kind and the bonding forces of the second kind (i.e. connecting a positive cell to the vessel wall).
321870
Centre National de Transfusion Sanguine
Gowling Lafleur Henderson Llp
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